Session 3 and session 4

Training School in Methodologies to study root-microbe interactions

Session 3

Genetic modification of microbes (bacteria) introduction of reporter genes (fluorescent or enzymatic tag, pro GUS, pro GFP…): chromosome insertion vs. plasmid, bombardment, heat shock, electroporation, conjugation; loss of function mutants: recombination, CRISPR-CAS9, transposon insertions…

Session 4

Microscopy: bright field – fluorescent – confocal – electronic. Staining of microorganisms,generation of reporter lines, immunolabeling

Toulouse, April 7th- 11th 2025